Previous videos:
Initiation:    • DNA Replication In Eukaryotes | Initi...  

Elongation:    • DNA Replication In Eukaryotes | Initi...  


In this video we have discussed the Termination of DNA replication in eukaryotes.Previously we had two lectures on initiation and elongation and now with this video we have completed the DNA replication in Eukaryotes.
The termination that we have discussed here has been seen in Xenopus species.

Eukaryotic DNA replication
Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.

DNA replication is the action of DNA polymerases synthesizing a DNA strand complementary to the original template strand. To synthesize DNA, the double-stranded DNA is unwound by DNA helicases ahead of polymerases, forming a replication fork containing two single-stranded templates. Replication processes permit the copying of a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. The major enzymatic functions carried out at the replication fork are well conserved from prokaryotes to eukaryotes, but the replication machinery in eukaryotic DNA replication is a much larger complex, coordinating many proteins at the site of replication, forming the replisome.

In termination we need the following proteins :
CMG Complex
Helicase /MCM complex
SCF Culin F box Protein
p97 protein / VCP protein /Segregase enzyme
K-48 ubiquitination
Lysine ubiquitation
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