g banding chromosomes - explains about the karyotyping process of chromosome. G-banding, G banding, or Giemsa banding is a technique used in cytogenetics to supply a noticeable karyotype by using staining condensed chromosomes. It's priceless for choosing genetic illnesses via the photographic representation of the complete chromosome complement. The metaphase chromosomes are handled with trypsin (to in part digest the chromosome) and stained with Giemsa stain. Heterochromatic regions, which are typically rich with adenine and thymine (AT-wealthy) DNA and quite gene-negative, stain more darkly in G-banding. In contrast, less condensed chromatin—which tends to be rich with guanine and cytosine (GC-rich) and more transcriptionally lively—contains less Giemsa stain, and these areas appear as mild bands in G-banding. The reverse of G‑bands is acquired in R‑banding. Banding can be utilized to establish chromosomal abnormalities, comparable to translocations, on the grounds that there's a specified sample of light and dark bands for each and every chromosome.

It's difficult to determine and team chromosomes centered on simple staining considering the uniform colour of the buildings makes it elaborate to distinguish between the special chromosomes. Hence, strategies like G‑banding have been developed that made "bands" show up on the chromosomes. These bands have been the same in look on the homologous chromosomes, as a consequence, identification grew to be less difficult and extra correct. The acid-saline-Giemsa protocol displays G-bands.
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