Once you’ve mastered the basics of DIY mushroom cultivation and you’re ready to take a deep dive into the heart of mycology, making your own agar media and petri dishes is a must.

In this in-depth video, you’ll discover the secret recipe to unlock infinite nutritious growth media for your fungus. Learn how to prepare, sterilize, pour, catalogue and store your very own petri dishes. Pick up lots of great tips and tricks along the way, including how to make hydrogen peroxide media (a great alternative to antibiotic agar), how to avoid senescence, get the most out of your mycelium and keep your fungus well-fed and happy for years to come.

Once you’ve tackled the techniques in this video, you’ll be ready to charge ahead into the uncharted territory of advanced DIY mycology, including breeding, cloning and culture preservation. Next, check out our tutorial on sterile culture technique, Mycology Lab 101:    • Mycology Lab 101: Agar Work, Cloning,...  

Made with love by Fungaia, a heart-centered, donation-based mushroom company:
https://www.fungaia.life/

For the best, most reliable and super affordable mushroom
genetics and liquid culture, visit our favorite culture library:

True Blue Genetics
https://www.TrueBlueGenetics.org/?cou...
Discount code: BLAGAR

____________________________

Supplies List

1. Mushroom Genetics

Gourmet and Medicinal Mushroom Cultures from Fungaia
https://www.fungaia.life/shop/species/

The best liquid culture on the market:
True Blue Genetics
https://truebluegenetics.org/?coupon=...
This is the last genetics provider you will ever need!

2. Ingredients

Organic Gelidium Agar & Media Premix
https://www.fungaia.life/shop/agar/

Malt Extract: https://amzn.to/3IX8LqA
Nutritional Yeast: https://amzn.to/3PD7sRa
Gypsum: https://amzn.to/3TBzyxt
Activated Charcoal: https://amzn.to/3TVTxIP

3. Petri Dishes

Sterile, Disposable Petri Dishes
Pack of 25: https://amzn.to/3IU0pQD
Case of 500: https://amzn.to/3PEVpmp

Pyrex Petri Dishes
https://amzn.to/4axywtq

____________________________

Recipes

MEA
10 grams agar
10 grams malt extract
pinch each gypsum & yeast
500 mL water

PDA
10 grams agar
8 grams dextrose
3 grams potato flakes
pinch each gypsum & yeast
500 mL water

VIDEO CORRECTION!
The correct dilution for hydrogen peroxide is 0.03%, not 0.3% as said in the video. The measurement shown is correct, 1 ml 3% H2O2 into 100 mL agar. Sorry!

____________________________

Other Useful Supplies

The Fungaia Cube Kit
https://www.fungaia.life/shop/kits/?q...
An organic, all-in-one grow kit for dung-lover mushrooms

Pre-Sterilized Grain Bags
https://www.fungaia.life/shop/fertile...

Pasteurized, Manure-Based Mushroom Substrate
https://www.fungaia.life/shop/fertile...

Monotubs and monotub filters: Microppose
https://microppose.com/r?id=h776jn
Discount code FUNGAIA

Bulk Liquid Culture IV Bags
https://www.fungaia.life/shop/spawn/?...

____________________________

More Videos from Fungaia

Mycology Lab 101: Sterile Culture Technique
   • Mycology Lab 101: Agar Work, Cloning,...  

How to Grow Mushrooms the Easy Way:
   • How to Grow Mushrooms from Scratch th...  

How to Make Grain Spawn:
   • How to Make Grain Spawn: A Deep Dive ...  

How to make your own liquid culture
   • How to Make Liquid Culture the Easy W...  

Advanced liquid culture method
   • How to Make Liquid Culture: DIY Mushr...  

How to make your own injection port jar lids:
   • How to Make Injection Port Jar Lids f...  

____________________________

Upcoming videos:

Introduction to Mushroom Cultivation
The Basics of Breeding
Storing Genetics and Managing a Mushroom Culture Library
Troubleshooting Contamination: Identification, Prevention and Problem-Solving
Working with Mushroom Grow Bags
Making Substrate for Dung-Loving Mushrooms
Making Substrate for Wood-Loving Mushrooms
Building a Home Lab
The Laminar Flow Hood
The Fungaia Flow Hood Kit
DIY Bulk Pasteurizer
DIY Cold Storage
DIY Water Filtration
Tyndallization: How to Make Grain Spawn without a Pressure Cooker
Low-Tech Mushroom Cultivation
Outdoor Mushroom Cultivation
The Liquid Culture Revolution: 5 Ways Liquid Culture Will Change the World
Fungaia's Bulk Liquid Culture System
R.E.M.I.S.: The Remarkably Efficient Mass Inoculation System
Myco-Materials

Created by Paul Lynn. All rights reserved.
(Made using some free stock footage.)
© 2024 Alembic Enterprises LLC
Walla Walla, Washington

0:00 Intro
3:18 Overview
3:35 Background
5:48 Side Note: No-Pour Agar Tek
6:26 Supplies
10:48 Ingredients
17:49 Recipes
23:05 Part 2: Prep & Sterilization
26:12 Part 3: Pouring Plates
29:14 Pro Tip #1: Color Coding
29:47 Pro Tip #2: Hydrogen Peroxide Plates
31:38 Drying
32:06 Pro Tip #3: Collating
32:34 Storage
33:44 Cleanup
34:08 Outro